The Core Histology Facility is supported by Orthopedic Research Biology. Its mission is to offer technical histologic and morphologic support for studies developing translational links between research and patient care. This lab provides support for the Department of Orthopedic Surgery Resident Research Projects, the orthopedic research projects of Drs. Hanley, Gruber, Sun, Patt, Tapp and Mauerhan involving intervertebral disc, cartilage, meniscus and adult-derived mesenchymal stem cells. The lab also supports other Cannon Research investigators and staff with their histology needs. The Core is directed by Helen Gruber, PhD with Jane Ingram, BS, a certified histology technician, as the Lab Supervisor. Mrs. Ingram brings expertise from many years in the clinical histopathology field and also experience in teaching histotechnology for certification. Natalia Zinchenko, BS, Research Technician III, provides expert technical support for this Core.
The Histology Core contains instrumentation to facilitate routine paraffin tissue embedding with a programmable paraffin infiltration unit, a paraffin embedding system, autostainer, and routine microtomes. Specialized non-decalcified bone histology is also performed using glycol methacrylate and methyl methacrylate with specialized hard tissue microtomes, including a large Polycut microtome. Expertise is offered in special staining, immunohistochemistry, immunocytochemistry, in situ hybridization, and cryo-sectioning techniques. A microscope room makes available to investigators and staff a Zeiss dual viewing microscope with brightfield, UV and polarized light capabilities. Digital imaging is available for brightfield and UV microscopy using a Nikon/Sony system which enables the user to capture and save digital images. Also available is a computer-based OsteoMeasure bone histomorphometry system for quantitative evaluation of bone and other tissues.
Helen E. Gruber, PhD, Director
Jane Ingram, BS, Lab Supervisor
Natalia Zinchenko, BS, Research Technician III
Khristina Bretthauer, Staff Assistant
Publications Resulting from Collaborations with Other Laboratories
Gruber HE, Ingram JA, Hanley, EN Jr. Morphologic complexity of the pericellular matrix in the annulus of the human intervertebral disc. Biotech Histochem 2007; 82: 217-225. [PMID: 18074268]
Gruber HE, Mougeot J-L, Hoelscher GL, Ingram JA, Hanley EN Jr. Microarray analysis of laser capture microdissected-annulus cells from the human intervertebral disc. Spine 2007; 32: 1181-1187. [PMID: 17495774]
Gruber HE, Ingram JA, Hoelscher G, Norton HJ, Hanley EN. Senescence in cells of the aging and degenerating intervertebral disc: Immunolocalization of senescence associated-ß-galactosidase in human and sand rat discs. Spine 2007; 32(3): 321-327. [PMID: 17268263]
Kurihara N, Hiruma Y, Zhou H, Subler MA, Dempster DW, Singer FR, Reddy SV, Gruber HE, Windle J, Roodman GD. Mutation of the sequestasome-1 gene (p62) increases osteoclastogenesis but does not induce Paget disease.J Clin Invest 2007; 117: 133-142. [PMID: 17187080]
Gruber HE, Ashraf N, Kilburn JM, Williams C, Norton HJ, Gordon BE, Hanley, E.N. Jr. Vertebral end plate architecture and vascularization: Application of micro-computerized tomography, a vascular tracer, and immunocytochemistry in analyses of disc degeneration in the aging sand rat. Spine 2005; 30: 2593-2600. [PMID: 16319744]
Gruber, HE, Ingram, J., Zinchenko, N., Hanley, EN. Practical Histologic Methodologies for Use with Cultured Cells. Biotechnic & Histochem. 84(6):283-286, 2009. [PMID: 19462311]
Gruber, H.E., Hanley, E.N., Sun, Y. Digital histologic images: practical pointers for successful electronic submission to biomedical journals. Biotechnic & Histochem. 84(6):295-298. 2009. [PMID: 19418314]