The Flow Cytometry Facility is housed in room 425 of Cannon Research Center. The facility is outfitted with two Becton Dickinson Flow Cytometers allowing both cell phenotyping and/or cell sorting.
The BD FACSCalibur Flow Cytometer meets the current demands of both clinical and research environments for cell phenotyping. The FACSCalibur's blue argon (488nm) and red (635nm) excitation lasers allow the concomitant analysis of up to 4-colors / 6 parameters (Forward and Size scatter) of cells.
This flow cytometer is controlled by a Macintosh computer using BD Cell Quest Pro data acquisition/analysis software. Additionally, FlowJo software is available for more detailed analysis.
BD FACSAria II
The recently purchased BD FACSAria II Flow Cytometer possesses, in addition to cell phenotyping, cell sorting capabilities. The FACSAria II is currently equipped with 2 excitation lasers (488nm and 635nm) and allows the detection of up to 7-colors / 9 parameter (Forward and Size Scatter) simultaneously for cell phenotyping. The FACSAria II also allows high speed / high purity cell sorting using up to 4-colors. This flow cytometer is controlled by a PC using BD Diva data acquisition software. FlowJo software is currently used for analysis and is compatible with both Mac and PC.
Iain McKillop, PhD, Group Director, General Surgery Labs
FACS Core Director
David Foureau, PhD, Postdoctoral Fellow, General Surgery Labs