The Cannon Confocal Microscopy and Imaging Core Facility (CCMICF) have acquired one of the most advanced microscopes for optical imaging. Our new state-of-the-art system is the Zeiss LSM 710 laser scanning confocal microscope. This system is built on AXIO Observer Z1 inverted microscope as the imaging platform. The Zeiss LSM 710 confocal microscope has excitation laser lines at 405, 458, 488, 514, 543 and 633nm. It has three separate spectral detection channels and is capable of detecting many standard fluorophores, including DAPI, CFP, GFP, fluorescein, rhodamine, texas red, RFP, CY5 and several of the AlexaFluor dyes. Suitable applications include imaging of single, double and triple-labeled specimens, immunohistochemistry, immunofluorescence, 3-dimensional image analysis, colocalization studies, time series, FRET and FRAP experiments. Image acquisition and processing are achieved using ZEN (Zeiss Efficient Navigation) software, a Windows-based program which offers significant improvements over the older LSM AIM software package. Specifically, ZEN allows easier analysis and improved features for ion concentration measurements, kinetic analysis, colocalization and image analysis.
The confocal system is housed in a temperature-controlled room on the second floor of Cannon Research Center in suite 234.
All users are required to fill out and submit a User Information Sheet prior to your scheduled appointment. We will use this information to set up the system for your session to ensure the best possible data acquisition for your study. It will significantly reduce your time needed on the system. Users are required to schedule their appointment at least one week in advance to avoid any unnecessary delay.
Sriparna Ghosh, PhD
Director, Confocal Microscopy and Imaging Core Facility
Research Scientist, LDMD
Cannon Research Center
Email: firstname.lastname@example.org or CHSConfocalMicroscopyFacility@carolinas.org