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Culture - duodenal tissue

Definition

A duodenal tissue culture is a laboratory exam to check a piece of tissue from the first part of the small intestine (duodenum) for infection-causing organisms.

Alternative Names

Duodenal tissue culture

How the test is performed

A piece of tissue from the first part of the small intestine is taken during an upper endoscopy (esophagogastroduodenoscopy).

The sample is then sent to a lab, and placed in a special dish (culture media) that allows bacteria or viruses to grow. The sample is placed under a microscope and checked at regular time periods to see if there are any organisms present and if they are growing.

Any organisms that grow on the culture are identified.

How to prepare for the test

This article discusses the culture test. For information on how to prepare for an upper endoscopy and biopsy procedure, see esophagogastroduodenoscopy.

Why the test is performed

A culture of duodenal tissue is done to check for bacteria that may lead to certain illnesses and conditions.

Normal Values

No harmful bacteria are found.

What abnormal results mean

An abnormal finding means that harmful bacteria has been found in the tissue sample. This may include organisms that cause inflammation of the stomach and intestines, such as:

  • Campylobacter
  • Helicobacter pylori
  • Salmonella

Special considerations

Usually other tests are done to identify infection-causing organisms in duodenal tissue. These tests include the urease test (for example the Clotest) and histology (looking at the tissue under the microscope).

Routine culture for H. pylori is not currently recommended.

References

DuPont HL. Approach to the patient with suspected enteric infection. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 291.

Semrad CE. Approach to the patient with diarrhea and malabsorption. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 142.

Giannella RA. Infectious enteritis and proctocolitis and bacterial food poisoning. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger & Fordtran's Gastrointestinal and Liver Disease. 9th ed. Philadelphia, Pa: Saunders Elsevier; 2010:chap 107.

Croft AC, Woods GL. Specimen collection and handling for diagnosis of infectious diseases. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 63.

Salwen MJ, Siddiqi HA, Gress FG, Bowne WB. Laboratory diagnosis of gastrointestinal and pancreatic disorders.

In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 22.

Fritsche R, Selvarangan R. Medical parasitology. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 62.


Review Date: 4/26/2012
Reviewed By: Linda J. Vorvick, MD, Medical Director and Director of Didactic Curriculum, MEDEX Northwest Division of Physician Assistant Studies, Department of Family Medicine, UW Medicine, University of Washington; and George F Longstreth, MD, Department of Gastroenterology, Kaiser Permanente Medical Care Program, San Diego, California. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M. Health Solutions, Ebix, Inc.
The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.
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