On day 1, urinate into the toilet when you get up in the morning.
Afterwards, collect all urine in a special container for the next 24 hours.
On day 2, urinate into the container when you get up in the morning.
Cap the container. Keep it in the refrigerator or a cool place during the collection period.
Label the container with your name, the date, the time of completion, and return it as instructed.
For an infant, thoroughly wash the area around the urethra. Open a urine collection bag (a plastic bag with an adhesive paper on one end), and place it on the infant. For males, place the entire penis in the bag and attach the adhesive to the skin. For females, place the bag over the labia. Diaper as usual over the secured bag.
This procedure may take a couple of attempts -- lively infants can move the bag, causing the urine to be absorbed by the diaper. The infant should be checked frequently and the bag changed after the infant has urinated into the bag. Drain the urine from the bag into the container provided by your health care provider.
Deliver it to the laboratory or your health care provider as soon as possible upon completion.
How to prepare for the test
Your health care provider will instruct you, if necessary, to stop taking any drugs that may interfere with the test results.
Drugs that can affect measurements include:
Nephrotoxic drugs (kidney damaging drugs)
The following may also interfere with test results.
Dye (contrast media) for a radiology exam within 3 days before the urine test
Severe emotional stress
Urinary tract infection
Urine contaminated with vaginal secretions
How the test will feel
The test involves only normal urination, and there is no discomfort.
Why the test is performed
Your doctor may order this test if you have signs or symptoms of glomerular disease, such as nephrotic syndrome, or another condition that may affect kidney function.
The normal value is less than 80 milligrams per day, or less than 10 milligrams per deciliter of urine.
The examples above are common measurements for results of these tests. Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.
Additional conditions under which the test may be performed:
Acute nephritic syndrome
Bodywide (systemic) infection (sepsis)
Hemolytic-uremic syndrome (HUS)
Medullary cystic kidney disease
Membranoproliferative GN I and GN II
Renal vein thrombosis
Healthy people may have higher than normal urine protein levels after strenuous exercise or with dehydration. Some foods may affect urine protein levels.
What the risks are
The test involves normal urination and there are no risks.
Sometimes, in order to avoid the inconvenience and possible inaccuracy of a 24-hour urine collection, your doctor may order a test done on just one urine sample, called the protein-to-creatinine ratio.
Landry DW, Bazari H. Approach to the patient with renal disease. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 116.
David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.